DrLupo held his third annual Build Against Cancer livestream on Twitch, raising over $2.3 million in just 24 hours. This brings his lifetime total to nearly $10 million, a record for “most money raised by a single creator.”

His livestream, which featured games like Fortnite, Minecraft, and Among Us, attracted a line of popular content creators and celebrities. Even Charlie, DrLupo’s son, helped raise $71,000 during a baking segment. In addition, Ninja jumped into a Fortnite battle with other creators, and others played games such as The Sims and Tetris99.

“We’re grateful for DrLupo, his family and fans who generously came together to give back to the patients and families at St. Jude Children’s Research Hospital and humbled by all the talented Twitch creators who gave their time and hearts to create an unimaginable 24-hour experience,” said Richard C. Shadyac Jr., President and CEO of ALSAC, the fundraising and awareness organization for St. Jude. “DrLupo’s dedication inspires us, and we’re thankful to have him and the Twitch community as a part of our St. Jude family.”

Co-hosts of the 24 hour event included Twitch Director of Creator Development djWHEAT, popular Twitch content creator and St. Jude supporter Bloodyfaster, and variety streamer and esports host GlitterXplosion. Celebrity appearances included Jordan Fisher, Robbie Amell, Mark Hoppus, Paul Scheer, Taran Killam, Felicia Day, Rahul Kohli, Mike Shinoda, Matt Mercer, Gary Whitta and Penny Arcade. Content creators taking part in the event include Ninja, NickMercs, CourageJD, BigCheeseKit, AnneMunition, lilsimsie, LuxieGames, Friskk and BrickinNick.

In addition, the “real Dr. Lupo” – Marybeth Lupo, Ph.D., a postdoctoral researcher at St. Jude Children’s Research Hospital, made an appearance to explain more about the research taking place at St. Jude. Ultimately, because of supporters like DrLupo, families at St. Jude will never receive a bill for treatment, travel, housing, or food.